DETERMINATION OF THE ORIENTATION DISTRIBUTION OF ADSORBED FLUOROPHORES USING TIRF .1. THEORY

被引：51

作者：

BOS, MA ^{[1
]}

KLEIJN, JM ^{[1
]}

机构：

[1] WAGENINGEN UNIV AGR,DEPT PHYS & COLLOID CHEM,6700 EK WAGENINGEN,NETHERLANDS

来源：

BIOPHYSICAL JOURNAL | 1995年 / 68卷 / 06期

关键词：

D O I：

10.1016/S0006-3495(95)80439-X

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

The spectroscopic technique total internal reflection fluorescence can be used for determination of the orientation of adsorbed fluorescent molecules. The underlying theory is presented in general terms and elaborated in detail for the case that the fluorescent group is a porphyrin ring. It is shown that order parameters of the orientation distribution can be obtained if both the fluorescence intensity and its polarization are measured as functions of the polarization of the incident laser beam. From these order parameters an approximation of the orientation distribution can be derived by the maximum-entropy method.

引用

页码：2566 / 2572

页数：7

共 32 条

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