DIFFERENTIAL RELEASE OF TUMOR-NECROSIS-FACTOR-ALPHA FROM MURINE PERITONEAL-MACROPHAGES STIMULATED WITH VIRULENT AND AVIRULENT SPECIES OF MYCOBACTERIA

被引:47
作者
FALCONE, V [1 ]
BASSEY, EB [1 ]
TONIOLO, A [1 ]
CONALDI, PG [1 ]
COLLINS, FM [1 ]
机构
[1] TRUDEAU INST INC,SARANAC LAKE,NY 12983
来源
FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY | 1994年 / 8卷 / 03期
关键词
MYCOBACTERIA; TUMOR NECROSIS FACTOR-ALPHA; VIRULENCE; MACROPHAGE;
D O I
10.1016/0928-8244(94)90054-X
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The ability of Mycobacterium tuberculosis H37Rv and H37Ra, M. bovis BCG and M. smegmatis to induce the secretion of tumor necrosis factor-alpha (TNF-alpha) by cultured murine peritoneal macrophages is inversely related to their virulence. The avirulent species of mycobacteria which were unable to persist in macrophages were capable of inducing significant levels of TNF-alpha compared to that formed in cultures infected with the virulent M. tuberculosis H37Rv. This difference was also associated with an inherent toxicity by live H37Rv for macrophage cultures. Heat-killed H37Rv was non-toxic and induced significant levels of TNF-alpha; in contrast, live and heat-killed suspensions of avirulent mycobacteria had an equivalent ability to trigger TNF-alpha secretion. The TNF-alpha response was dose-dependent, related directly to the percentage of infected cells, and peaked 6-12 h post-infection. An early and vigorous TNF-alpha response appears to be a marker of macrophage resistance, while the downregulation of this response seems associated with macrophage toxicity and unrestricted mycobacterial growth.
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页码:225 / 232
页数:8
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