3β-Acetoxychol-5-enoic-24-14C acid was administered to a rat with a bile fistula. Bile samples were collected, and the following natural and unnatural bile acids were identified as metabolites: 3α- and 3β-hydroxy-cholanoic acids of the 5β- and 5α-series; 3e{open}, 6e{open}-dihydroxycholanoic acids of the 5β- and 5α-series which were identified as 3, 6-dioxo-5β-cholanoic and 3, 6-dioxo-5α-cholanoic acids after oxidation; 3α, 7α-, 3α, 7β- and possibly 3β, 7e{open}-dihydroxy-5β-cholanoic acids. From this result a tentative pathway leading to those metabolites is presented. © 1968 by the Journal of Biochemistry.
