DEVELOPMENTAL EXPRESSION, SYNTHESIS, AND SECRETION OF INSECTICYANIN BY THE EPIDERMIS OF THE TOBACCO HORNWORM, MANDUCA-SEXTA

被引：39

作者：

RIDDIFORD, LM ^{[1
]}

PALLI, SR ^{[1
]}

HIRUMA, K ^{[1
]}

LI, W ^{[1
]}

GREEN, J ^{[1
]}

HICE, RH ^{[1
]}

WOLFGANG, WJ ^{[1
]}

WEBB, BA ^{[1
]}

机构：

[1] UNIV CALIF BERKELEY,DEPT GENET,BERKELEY,CA 94720

来源：

ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY | 1990年 / 14卷 / 03期

关键词：

D O I：

10.1002/arch.940140305

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Insecticyanin was found to be synthesized in several isoelectric forms and stored in the pigment granules in the epidermis. Both major epidermal forms (INS‐a, pl 5.5; INS‐b, pl 5.7) were found in the cuticle, but only the most basic form, INS‐b, was present in the hemolymph. In vitro the epidermis synthesized and secreted both forms into both the cuticle and the medium. Isolation of two cDNA clones for insecticyanin followed by hybridization to epidermal mRNA showed the presence of only one 1.1 kb mRNA, but transcription of the longer cDNA yielded a RNA which produced INS‐a but no INS‐b. Insecticyanin mRNA was present during the intermolt feeding stages of the 4th and 5th instars and absent during the larval molt and after the onset of metamorphosis. Exposure of either day 2 4th‐instar or day 1 5th‐instar larval epidermis to 20‐hydroxyecdysone (20HE) in vitro caused a dose‐dependent decline in this mRNA that was not prevented by simultaneous exposure to JH. When synthesis resumes just before ecdysis, INS‐b appears before INS‐a; then on the final day of feeding, synthesis of INS‐a ceases before that of INS‐b. Copyright © 1990 Wiley‐Liss, Inc.

引用

页码：171 / 190

页数：20

共 38 条

[31] CORRELATIONS BETWEEN EPIDERMAL-CELL STRUCTURE AND ENDOGENOUS HORMONE TITERS DURING THE 5TH LARVAL INSTAR OF THE TOBACCO HORNWORM, MANDUCA-SEXTA [J].