MICROINJECTION OF CULTURED RAT EMBRYOS - STUDIES WITH RETINOL, 13-CIS-RETINOIC AND ALL-TRANS-RETINOIC ACID

被引：25

作者：

LEE, QP ^{[1
]}

JUCHAU, MR ^{[1
]}

KRAFT, JC ^{[1
]}

机构：

[1] UNIV WASHINGTON,SCH MED,DEPT PHARMACOL SJ30,SEATTLE,WA 98195

来源：

TERATOLOGY | 1991年 / 44卷 / 03期

关键词：

D O I：

10.1002/tera.1420440308

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Retinol, all-trans-retinoic acid or 13-cis-retinoic acid were intraamniotically microinjected in rat embryos on day 10 of gestation and cultured until day 11.5. A comparison of the concentration-effect relationships of the retinoids showed that the dysmorphogenic effects were qualitatively similar for all three, but were elicited by a low concentration of all-trans-retinoic acid (250 ng/ml), a 6- to 7-fold higher concentration of 13-cis-retinoic acid and an approximately 16-fold higher concentration of retinol. After microinjection of 2,000 ng/ml of retinol, no dysmorphogenesis was observed but instead an increase in all growth parameters as compared to the controls.

引用

页码：313 / 323

页数：11

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