HETEROMERIC NMDA RECEPTORS - MOLECULAR AND FUNCTIONAL DISTINCTION OF SUBTYPES

被引:2303
作者
MONYER, H
SPRENGEL, R
SCHOEPFER, R
HERB, A
HIGUCHI, M
LOMELI, H
BURNASHEV, N
SAKMANN, B
SEEBURG, PH
机构
[1] UNIV HEIDELBERG, CTR MOLEC BIOL, NEUENHEIMER FELD 282, W-6900 HEIDELBERG, GERMANY
[2] MAX PLANCK INST MED RES, W-6900 HEIDELBERG 1, GERMANY
关键词
D O I
10.1126/science.256.5060.1217
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The N-methyl D-aspartate (NMDA) receptor subtype of glutamate-gated ion channels possesses high calcium permeability and unique voltage-dependent sensitivity to magnesium and is modulated by glycine. Molecular cloning identified three complementary DNA species of rat brain, encoding NMDA receptor subunits NMDAR2A (NR2A), NR2B, and NR2C, which are 55 to 70% identical in sequence. These are structurally related, with less than 20% sequence identity to other excitatory amino acid receptor subunits, including the NMDA receptor subunit NMDAR1 (NR1). Upon expression in cultured cells, the new subunits yielded prominent, typical glutamate- and NMDA-activated currents only when they were in heteromeric configurations with NR1. NR1-NR2A and NR1-NR2C channels differed in gating behavior and magnesium sensitivity. Such heteromeric NMDA receptor subtypes may exist in neurons, since NR1 messenger RNA is synthesized throughout the mature rat brain, while NR2 messenger RNA show a differential distribution.
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页码:1217 / 1221
页数:5
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