INTERNAL RIBOSOME ENTRY SITE OF ENCEPHALOMYOCARDITIS VIRUS-RNA IS UNABLE TO DIRECT TRANSLATION IN SACCHAROMYCES-CEREVISIAE

被引：21

作者：

EVSTAFIEVA, AG

BELETSKY, AV

BOROVJAGIN, AV

BOGDANOV, AA

机构：

[1] A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University

来源：

FEBS LETTERS | 1993年 / 335卷 / 02期

关键词：

TRANSLATION; INTERNAL INITIATION; ENCEPHALOMYOCARDITIS VIRUS; PROTHYMOSIN A; SACCHAROMYCES CEREVISIAE;

D O I：

10.1016/0014-5793(93)80745-G

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To evaluate the potential of the encephalomyocarditis virus (EMCV) internal ribosome entry site ORES) to promote efficient expression of foreign genes in the yeast, S. cerevisiae, we have constructed E. coli-yeast shuttle Vectors in which the EMCV 5' non-coding region was fused to the reporter gene, human prothymosin alpha. Efficiency of translation of corresponding RNA transcripts in mammalian cell-free systems was highly dependent on the sequence context and/or position of the initiation codon. No translation of these IRES-dependent mRNAs occurred in S. cerevisiae.

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页码：273 / 276

页数：4

相关论文

共 21 条

[1] INTERNAL INITIATION OF TRANSLATION IN RETROVIRAL VECTORS CARRYING PICORNAVIRUS-5' NONTRANSLATED REGIONS [J].

ADAM, MA ;

RAMESH, N ;

MILLER, AD ;

OSBORNE, WRA .

JOURNAL OF VIROLOGY, 1991, 65 (09) :4985-4990

[2] TRANSLATION INITIATION FACTOR-DEPENDENT EXTRACTS FROM SACCHAROMYCES-CEREVISIAE [J].

ALTMANN, M ;

BLUM, S ;

PELLETIER, J ;

SONENBERG, N ;

WILSON, TMA ;

TRACHSEL, H .

BIOCHIMICA ET BIOPHYSICA ACTA, 1990, 1050 (1-3) :155-159

[3] A FACTOR THAT SPECIFICALLY BINDS TO THE 5'-UNTRANSLATED REGION OF ENCEPHALOMYOCARDITIS VIRUS-RNA [J].

BOROVJAGIN, AV ;

EVSTAFIEVA, AG ;

UGAROVA, TY ;

SHATSKY, IN .

FEBS LETTERS, 1990, 261 (02) :237-240

[4]

CIGAN AM, 1987, GENE, V59, P1

[5] YEAST-CELLS ARE INCAPABLE OF TRANSLATING RNAS CONTAINING THE POLIOVIRUS 5' UNTRANSLATED REGION - EVIDENCE FOR A TRANSLATIONAL INHIBITOR [J].

COWARD, P ;

DASGUPTA, A .

JOURNAL OF VIROLOGY, 1992, 66 (01) :286-295

[6] SYNTHESIS OF FUNCTIONAL-MOUSE CYTOCHROME-P-450-P1 AND CHIMERIC CYTOCHROME-P-450-P3-1 IN THE YEAST SACCHAROMYCES-CEREVISIAE [J].

CULLIN, C ;

POMPON, D .

GENE, 1988, 65 (02) :203-217

[7] THE SEQUENCE CONTEXT OF THE INITIATION CODON IN THE ENCEPHALOMYOCARDITIS VIRUS LEADER MODULATES EFFICIENCY OF INTERNAL TRANSLATION INITIATION [J].

DAVIES, MV ;

KAUFMAN, RJ .

JOURNAL OF VIROLOGY, 1992, 66 (04) :1924-1932

[8] SEQUENCE AND STRUCTURAL ELEMENTS THAT CONTRIBUTE TO EFFICIENT ENCEPHALOMYOCARDITIS VIRUS-RNA TRANSLATION [J].

DUKE, GM ;

HOFFMAN, MA ;

PALMENBERG, AC .

JOURNAL OF VIROLOGY, 1992, 66 (03) :1602-1609

[9] CAP-INDEPENDENT TRANSLATION OF MESSENGER-RNA CONFERRED BY ENCEPHALOMYOCARDITIS VIRUS 5' SEQUENCE IMPROVES THE PERFORMANCE OF THE VACCINIA VIRUS BACTERIOPHAGE-T7 HYBRID EXPRESSION SYSTEM [J].

ELROYSTEIN, O ;

FUERST, TR ;

MOSS, B .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (16) :6126-6130

[10] A COMPLEX RNA SEQUENCE DETERMINES THE INTERNAL INITIATION OF ENCEPHALOMYOCARDITIS VIRUS-RNA TRANSLATION [J].

EVSTAFIEVA, AG ;

UGAROVA, TY ;

CHERNOV, BK ;

SHATSKY, IN .

NUCLEIC ACIDS RESEARCH, 1991, 19 (03) :665-671