EFFECTIVE ONLINE COUPLING OF HPLC AND FLAME-AAS BY MEANS OF HYDRAULIC HIGH-PRESSURE NEBULIZATION

被引：32

作者：

WEBER, G

BERNDT, H

机构：

[1] Institut für Spektrochemie und angewandte Spektroskopie, Dortmund 1, D-4600

来源：

CHROMATOGRAPHIA | 1990年 / 29卷 / 5-6期

关键词：

Column liquid chromatography; Hydraulic high pressure nebulization; On-line flame-AAS detection; Trace metal speciation;

D O I：

10.1007/BF02317914

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Hydraulic high pressure nebulization is used as an effective way of on-line coupling of HPLC to flame-AAS for speciation of metal compounds in the ng range. Compared to coupling with a conventional nebulizer a signal enhancement by a factor of 7.5 (peak height) and 10.1 (peak area) for copper is obtained. Using an injected volume of 50 μL the detection limits for Cu, Fe, Ni and Cd are below 0.1 μg/mL (=5ng) and for Mg below 0.01 μg/mL (=0.5ng). The effects of HPLC flow-rate and nebulization nozzle diameter on the signal peak height have been investigated. The performance of the system is demonstrated using speciation of iron. A base-line separation of Fe(II) and Fe(III) is achieved within two minutes. Also species changes (Fe acetate to Fe citrate) can be analysed using the proposed system. © 1990 Friedr. Vieweg & Sohn Verlagsgesellschaft mbH.

引用

页码：254 / 258

页数：5

共 14 条

[11] CITRATE BINDING OF AL-3+ AND FE-3+ [J].