STABLE TRANSFECTION OF CALBINDIN-D28K INTO THE GH3 CELL-LINE ALTERS CALCIUM CURRENTS AND INTRACELLULAR CALCIUM HOMEOSTASIS

被引:180
作者
LLEDO, PM
SOMASUNDARAM, B
MORTON, AJ
EMSON, PC
MASON, WT
机构
[1] INST ANIM PHYSIOL & GENET RES,MED RES COUNCIL GRP,CAMBRIDGE CB2 4AT,ENGLAND
[2] INST ANIM PHYSIOL & GENET RES,AGR FOOD RES COUNCIL,CAMBRIDGE CB2 4AT,ENGLAND
基金
英国惠康基金;
关键词
D O I
10.1016/0896-6273(92)90246-A
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Previous work demonstrating the presence and differential distribution of Ca2+-binding proteins in the CNS has led to the proposal that cytosolic proteins, such as calbindin-D28k (CB), may play a pivotal role in neurons. We have used a retrovirus containing the full-length cDNA for CB to transfect the pituitary tumor cell line GH3, to generate CB-expressing GH3 cells and to investigate whether ionic channel activities as well as the concentration of intracellular free Ca2+ ([Ca2+]i) homeostasis could be altered by the presence of this Ca2+-binding protein. We show that CB-transfected GH3 cells exhibited lower Ca2+ entry through voltage-dependent Ca2+ channels and were better able to reduce [Ca2+]i transients evoked by voltage depolarizations than the wild-type parent cell line. These observations provide a mechanism by which CB may protect tissues against Ca2+-mediated excitotoxicity.
引用
收藏
页码:943 / 954
页数:12
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