EVIDENCE FOR ENDOTHELIN-INDUCED RENAL VASOCONSTRICTION INDEPENDENT OF ETA RECEPTOR ACTIVATION

被引:167
作者
POLLOCK, DM
OPGENORTH, TJ
机构
来源
AMERICAN JOURNAL OF PHYSIOLOGY | 1993年 / 264卷 / 01期
关键词
ENDOTHELIN RECEPTORS; ENDOTHELIAL CELLS; RENAL HEMODYNAMICS; KIDNEY;
D O I
10.1152/ajpregu.1993.264.1.R222
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
Experiments were designed to examine the role of endothelin (ET) receptors, specifically ETA receptors, in mediating the renal vasoconstrictor effects of ET-1 in anesthetized Sprague-Dawley rats. Intravenous infusion of ET-1 at 25 pmol.kg-1.min-1 for 60 min produced a significant increase in mean arterial pressure (20 +/-7%) and decreases in renal plasma flow (-60 +/- 6%) and glomerular filtration rate (-47 +/- 6%). Renal vascular resistance was significantly increased from 17 +/- 1 mmHg.ml-1.min.g kidney wt during control period to 54 +/- 11 mmHg.ml-1.min.g kidney wt during the experimental period. A second group of rats was infused with both ET-1 and the specific ETA receptor antagonist BQ-123 (0.1 mg.kg-1.min-1). ET-1-induced increases in mean arterial pressure were completely blocked by BQ-123 (the average change was -7 +/- 4%). However, the renal vasoconstrictor effects of ET-1 were not affected by the antagonist, since renal plasma flow and glomerular filtration rate were again significantly reduced (-54 +/- 4 and -56 +/- 6%, respectively). Once again, renal vascular resistance was significantly increased from 16 +/- 2 mmHg.ml-1.min-g kidney wt during the control period to 33 +/- 5 mmHg.ml-1.min.g kidney wt during the experimental period. In a third group, infusion of BQ-123 alone produced a significant decline in mean arterial pressure (-13 +/- 2%), with no significant changes in renal plasma flow or glomerular filtration rate, thus producing a significant decrease in renal vascular resistance (15 +/- 1 vs. 11 +/- 2 mmHg.ml-1.min.g kidney wt). These results suggest that ET-induced renal vasoconstriction is not mediated by ETA receptors and that ETA receptor activation may play a role in maintaining arterial pressure and renal vascular resistance in normal anesthetized rats.
引用
收藏
页码:R222 / R226
页数:5
相关论文
共 30 条
  • [1] CLONING AND EXPRESSION OF A CDNA-ENCODING AN ENDOTHELIN RECEPTOR
    ARAI, H
    HORI, S
    ARAMORI, I
    OHKUBO, H
    NAKANISHI, S
    [J]. NATURE, 1990, 348 (6303) : 730 - 732
  • [2] CHITTINANDANA A, 1991, Journal of the American Society of Nephrology, V2, P644
  • [3] SPECIFIC RECEPTORS FOR ENDOTHELIN-3 IN CULTURED BOVINE ENDOTHELIAL-CELLS AND ITS CELLULAR MECHANISM OF ACTION
    EMORI, T
    HIRATA, Y
    MARUMO, F
    [J]. FEBS LETTERS, 1990, 263 (02) : 261 - 264
  • [4] FIKURODA T, 1992, LIFE SCI, V50, pP107
  • [5] CARDIOVASCULAR, RENAL, AND ENDOCRINE RESPONSES TO INTRAVENOUS ENDOTHELIN IN CONSCIOUS DOGS
    GOETZ, KL
    WANG, BC
    MADWED, JB
    ZHU, JL
    LEADLEY, RJ
    [J]. AMERICAN JOURNAL OF PHYSIOLOGY, 1988, 255 (06): : R1064 - R1068
  • [6] LOCALIZATION AND QUANTIFICATION OF [I-125] ENDOTHELIN BINDING-SITES IN HUMAN FETAL AND ADULT KIDNEYS - RELEVANCE TO RENAL ONTOGENY AND PATHOPHYSIOLOGY
    GRONE, HJ
    LAUE, A
    FUCHS, E
    [J]. KLINISCHE WOCHENSCHRIFT, 1990, 68 (15): : 758 - 767
  • [7] HEMODYNAMIC AND RENAL TUBULAR EFFECTS OF LOW-DOSES OF ENDOTHELIN IN ANESTHETIZED RATS
    HARRIS, PJ
    ZHUO, J
    MENDELSOHN, FAO
    SKINNER, SL
    [J]. JOURNAL OF PHYSIOLOGY-LONDON, 1991, 433 : 25 - 39
  • [8] RENAL VASOCONSTRICTION BY THE ENDOTHELIAL-CELL DERIVED PEPTIDE ENDOTHELIN IN SPONTANEOUSLY HYPERTENSIVE RATS
    HIRATA, Y
    MATSUOKA, H
    KIMURA, K
    FUKUI, K
    HAYAKAWA, H
    SUZUKI, E
    SUGIMOTO, T
    SUGIMOTO, T
    YANAGISAWA, M
    MASAKI, T
    [J]. CIRCULATION RESEARCH, 1989, 65 (05) : 1370 - 1379
  • [9] BIOLOGICAL PROFILES OF HIGHLY POTENT NOVEL ENDOTHELIN ANTAGONISTS SELECTIVE FOR THE ETA RECEPTOR
    IHARA, M
    NOGUCHI, K
    SAEKI, T
    FUKURODA, T
    TSUCHIDA, S
    KIMURA, S
    FUKAMI, T
    ISHIKAWA, K
    NISHIKIBE, M
    YANO, M
    [J]. LIFE SCIENCES, 1992, 50 (04) : 247 - 255
  • [10] AN ENDOTHELIN RECEPTOR (ETA) ANTAGONIST ISOLATED FROM STREPTOMYCES-MISAKIENSIS
    IHARA, M
    FUKURODA, T
    SAEKI, T
    NISHIKIBE, M
    KOJIRI, K
    SUDA, H
    YANO, M
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1991, 178 (01) : 132 - 137