ANTHOCYANIN RELEASE FROM GRAPE (VITIS-VINIFERA L) CELL-SUSPENSION

被引：10

作者：

CORMIER, F ^{[1
]}

DO, CB ^{[1
]}

MORESOLI, C ^{[1
]}

ARCHAMBAULT, J ^{[1
]}

CHAVARIE, C ^{[1
]}

CHAOUKI, F ^{[1
]}

PEPIN, MF ^{[1
]}

机构：

[1] ECOLE POLYTECH,DEPT CHEM ENGN,MONTREAL H3C 3A7,QUEBEC,CANADA

来源：

BIOTECHNOLOGY LETTERS | 1992年 / 14卷 / 11期

关键词：

D O I：

10.1007/BF01021053

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In order to develop a protocol for the release of intravacuolarly-stored anthocyanins from grapevine (Vitis vinifera L.) cell suspension with preserved viability, the effects of extracellular pH modifications, of solid adsorbents in the culture medium (two-phase system) and of chemical permeabilization were investigated. The solid adsorbent Amberlite IR-120 and chemical permeabilization were efficient in enabling the release of up to 70-80% of vacuolar anthocyanins. However, the release was always at the expense of a proportionate loss in cell viability. Results demonstrate the irreversible nature of anthocyanin sequestration.

引用

页码：1029 / 1034

页数：6

共 24 条

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