FORMYLMETHANOFURAN DEHYDROGENASES FROM METHANOGENIC ARCHAEA - SUBSTRATE-SPECIFICITY, EPR PROPERTIES AND REVERSIBLE INACTIVATION BY CYANIDE OF THE MOLYBDENUM OR TUNGSTEN IRON-SULFUR PROTEINS

被引:52
作者
BERTRAM, PA
KARRASCH, M
SCHMITZ, RA
BOCHER, R
ALBRACHT, SPJ
THAUER, RK
机构
[1] MAX PLANCK INST TERR MIKROBIOL,D-35043 MARBURG,GERMANY
[2] UNIV MARBURG,FACHBEREICHS BIOL PHILIPPS,MIKROBIOL LAB,MARBURG,GERMANY
[3] UNIV AMSTERDAM,EC SLATER INST BIOCHEM RES,AMSTERDAM,NETHERLANDS
来源
EUROPEAN JOURNAL OF BIOCHEMISTRY | 1994年 / 220卷 / 02期
关键词
D O I
10.1111/j.1432-1033.1994.tb18646.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Formylmethanofuran dehydrogenases, which are found in methanogenic Archaea, are molybdenum or tungsten iron-sulfur proteins containing a pterin cofactor. We report here on differences in substrate specificity, EPR properties and susceptibility towards cyanide inactivation of the enzymes from Methanosarcina barkeri, Methanobacterium thermoautotrophicum and Methanobacterium wolfei. The molybdenum enzyme from M. barkeri (relative activity with N-formylmethanofuran = 100%) was found to catalyze, albeit at considerably reduced apparent V-max, the dehydrogenation of N-furfurylformamide (11%), N-methylformamide (0.2%), formamide (0.1%) and formate (1%). The molybdenum enzyme from M. wolfei could only use N-furfurylformamide (1%) and formate (3%) as pseudosubstrates. The molybdenum enzyme from M. thermoautotrophicum and the tungsten enzymes from M. thermoautotrophicum and M. wolfei were specific for N-formylmethanofuran. The molybdenum formylmethanofuran dehydrogenases exhibited at 77 K two rhombic EPR signals, designated FMD(red) and FMD(ox), both derived from Mo as shown by isotopic substitution with Mo-97. The FMD(red) signal was only displayed by the active enzyme in the reduced form and was lost upon enzyme oxidation; the FMD(ox) signal was displayed by an inactive form and was not quenched by O-2. The tungsten isoenzymes were EPR silent. The molybdenum formylmethanofuran dehydrogenases were found to be inactivated by cyanide whereas the tungsten isoenzymes, under the same conditions, were not inactivated. Inactivation was associated with a characteristic change in the molybdenum-derived EPR signal. Reactivation was possible in the presence of sulfide.
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页码:477 / 484
页数:8
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