MOLYBDOPTERIN ADENINE-DINUCLEOTIDE AND MOLYBDOPTERIN HYPOXANTHINE DINUCLEOTIDE IN FORMYLMETHANOFURAN DEHYDROGENASE FROM METHANOBACTERIUM-THERMOAUTOTROPHICUM (MARBURG)

被引:71
作者
BORNER, G [1 ]
KARRASCH, M [1 ]
THAUER, RK [1 ]
机构
[1] UNIV MARBURG,FACHBEREICH BIOL,MIKROBIOL LAB,KARL VON FRISCH STR,W-3550 MARBURG,GERMANY
关键词
MOLYBDOPTERIN COFACTOR; MOLYBDOENZYME; FORMYLMETHANOFURAN DEHYDROGENASE; ARCHAEBACTERIUM; METHANOGENIC BACTERIUM;
D O I
10.1016/0014-5793(91)81218-W
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Formylmethanofuran dehydrogenase from Methanobacterium thermoautotrophicum was purified to apparent homogeneity and found to contain per mol (apparent molecular mass 110 kDa) 0.6 mol molybdenum, 4 mol non-heme iron, 4 mol acid-labile sulfur, and in addition, 0.7 mol of a pterin-containing co-factor (apparent molecular mass 800 Da) which has been characterized. The pterin material was extracted after alkylation by iodoacetamide and the extract subjected to HPLC on Lichrospher 100 RP-18. Three pterin compounds were resolved. On the basis of their UV/visible spectra and of the products formed after cleavage by nucleotide pyrophosphatase and alkaline phosphatase they were identified as the [di(carboxamidomethyl)]-derivatives of molybdopterin guanine dinucleotide (MGD), of molybdopterin adenine dinucleotide (MAD), and of molybdopterin hypoxanthine dinucleotide (MHD). The three pterin dinucleotides were present in the proportions 1:0:4:0.1.
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页码:31 / 34
页数:4
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