MOLYBDOPTERIN ADENINE-DINUCLEOTIDE AND MOLYBDOPTERIN HYPOXANTHINE DINUCLEOTIDE IN FORMYLMETHANOFURAN DEHYDROGENASE FROM METHANOBACTERIUM-THERMOAUTOTROPHICUM (MARBURG)

被引：71

作者：

BORNER, G ^{[1
]}

KARRASCH, M ^{[1
]}

THAUER, RK ^{[1
]}

机构：

[1] UNIV MARBURG,FACHBEREICH BIOL,MIKROBIOL LAB,KARL VON FRISCH STR,W-3550 MARBURG,GERMANY

来源：

FEBS LETTERS | 1991年 / 290卷 / 1-2期

关键词：

MOLYBDOPTERIN COFACTOR; MOLYBDOENZYME; FORMYLMETHANOFURAN DEHYDROGENASE; ARCHAEBACTERIUM; METHANOGENIC BACTERIUM;

D O I：

10.1016/0014-5793(91)81218-W

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Formylmethanofuran dehydrogenase from Methanobacterium thermoautotrophicum was purified to apparent homogeneity and found to contain per mol (apparent molecular mass 110 kDa) 0.6 mol molybdenum, 4 mol non-heme iron, 4 mol acid-labile sulfur, and in addition, 0.7 mol of a pterin-containing co-factor (apparent molecular mass 800 Da) which has been characterized. The pterin material was extracted after alkylation by iodoacetamide and the extract subjected to HPLC on Lichrospher 100 RP-18. Three pterin compounds were resolved. On the basis of their UV/visible spectra and of the products formed after cleavage by nucleotide pyrophosphatase and alkaline phosphatase they were identified as the [di(carboxamidomethyl)]-derivatives of molybdopterin guanine dinucleotide (MGD), of molybdopterin adenine dinucleotide (MAD), and of molybdopterin hypoxanthine dinucleotide (MHD). The three pterin dinucleotides were present in the proportions 1:0:4:0.1.

引用

页码：31 / 34

页数：4