GAS-CHROMATOGRAPHIC MASS-SPECTROMETRIC QUANTITATION OF TRIMETHYLXANTHINES, DIMETHYLXANTHINES AND MONOMETHYLXANTHINES AND URIC ACIDS FROM HEPATOCYTE INCUBATION MEDIA

被引：8

作者：

BENCHEKROUN, Y ^{[1
]}

DESAGE, M ^{[1
]}

RIBON, B ^{[1
]}

BRAZIER, JL ^{[1
]}

机构：

[1] FAC PHARM LYON, ETUD ANALYT & CINET MED LAB, 8 AVE ROCKEFELLER, F-69373 Lyon 08, FRANCE

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1990年 / 532卷 / 02期

关键词：

D O I：

10.1016/S0378-4347(00)83777-9

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A gas chromatographic-mass spectrometric method is described for the measurement of the concentration of fourteen methylxanthines and methyluric acid metabolites of methylxanthines, especially caffeine, from cell incubation media. The method shows linearity, accuracy and recovery suitable for metabolic studies. The reproducibility of relative retention times is satisfactory (0.07%) and allows rapid and conclusive identification of chromatographic peaks corresponding to metabolites. Moreover, this method enables the simultaneous determination of 3,7-methylxanthine and its 1,7-isomer, which are not chromatographically resolved. This method can be successfully applied when molecules labelled with stable isotopes are used as tracers for metabolic studies. © 1990.

引用

页码：261 / 275

页数：15

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