GLUTATHIONE CONJUGATION OF MICROSOME-MEDIATED AND SYNTHETIC AFLATOXIN-B1-8,9-OXIDE BY PURIFIED GLUTATHIONE S-TRANSFERASES FROM RATS

被引：38

作者：

GOPALAN, P

JENSEN, DE

LOTLIKAR, PD

机构：

[1] TEMPLE UNIV,HLTH SCI CTR,SCH MED,FELS INST CANC RES & MOLEC BIOL,PHILADELPHIA,PA 19140

[2] THOMAS JEFFERSON UNIV,SCH MED,DEPT PHARMACOL,PHILADELPHIA,PA 19107

来源：

CANCER LETTERS | 1992年 / 64卷 / 03期

关键词：

AFLATOXIN-B1; AFLATOXIN-B1-EPOXIDE; AFLATOXIN-B1-GLUTATHIONE; RAT GLUTATHIONE TRANSFERASES;

D O I：

10.1016/0304-3835(92)90047-Y

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Glutathione (GSH) conjugation of microsome-mediated and synthetic aflatoxin B1 (AFB1)-epoxide and styrene oxide has been investigated with purified GSH S-transferases (GSTs) from rats. Both styrene oxide and AFB1-epoxide were conjugated preferentially by m-mu GSTs 3-3, 3-4 and 4-4 as compared to alpha GSTs 1-1, 1-2 and 2-2. The highest catalytic activity with styrene oxide conjugation was associated with GST 4-4. The highest catalytic activity with microsome-mediated AFB1-epoxide conjugation was observed with GST 3-3 whereas with the synthetic AFB1-epoxide conjugation was seen with GST 4-4. The catalytic activity of pi GST 7-7 was intermediate to m-mu and alpha GSTs. It is suggested that GST 3-3 may play an important role in inactivation of AFB1-epoxide generated in vivo in the rat.

引用

页码：225 / 233

页数：9

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