ELECTROPHORETICALLY MEDIATED MICROANALYSIS OF CALCIUM

被引：36

作者：

PATTERSON, DH ^{[1
]}

HARMON, BJ ^{[1
]}

REGNIER, FE ^{[1
]}

机构：

[1] PURDUE UNIV,DEPT CHEM,W LAFAYETTE,IN 47907

来源：

JOURNAL OF CHROMATOGRAPHY A | 1994年 / 662卷 / 02期

关键词：

D O I：

10.1016/0021-9673(94)80526-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Electrophetically mediated microanalysis (EMMA) was used for the determination of calcium. The faster analyte zone containing the calcium was injected spatially behind a slower zone of o-cresolphthalein complexone in a capillary electrophoresis based system. Upon application of the electric field the calcium zone was electrophoretically mixed with the reagent and product was formed. The bulk electroosmotic flow carried the product to the detector where the absorbance of the resulting complex at 575 nm was measured. Quantitation using an internal standard yielded a linear response with an R.S.D. of 8.1%. An inter-method comparison was performed with the standard bulk method and yielded results that did not significantly differ. The advantages of EMMA with respect to traditional methods were addressed.

引用

页码：389 / 395

页数：7

共 18 条

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