YEAST CONTAMINANTS OF MICROPROPAGATED PLANT CULTURES

被引：14

作者：

LEIFERT, C ^{[1
]}

WAITES, WM ^{[1
]}

NICHOLAS, JR ^{[1
]}

KEETLEY, JWW ^{[1
]}

机构：

[1] NEOPLANTS LTD, FRECKLETON PR4 1HU, LANCS, ENGLAND

来源：

JOURNAL OF APPLIED BACTERIOLOGY | 1990年 / 69卷 / 04期

关键词：

D O I：

10.1111/j.1365-2672.1990.tb01537.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Leifert, C., Waites, W.M., Nicholas, J.R. & Keetley, J.W. 1990. Yeast contaminants of micropropagated plant cultures. Journal of Applied Bacteriology69, 471–476. Of 36 yeast strains isolated from contaminated plant cultures 78% were Candida, 20%Rhodotorula and 2% were Cryptococcus species. Strains of Candida guilliermon‐dii represented 45% of all yeasts isolated. Yeasts grew rapidly on plant growth media with a pH of between 2.5 and 6.0 and decreased the medium pH to values of between 2.0 and 3.0. Candida yeasts growing on plant medium for 28 d metabolized about 75% of the sucrose and produced fermentation products such as ethanol and acetic acid. In contrast, Rhodotorula did not produce ethanol or acetic acid and only metabolized about 20% of the sucrose. Possible sources of contamination are discussed. Copyright © 1990, Wiley Blackwell. All rights reserved

引用

页码：471 / 476

页数：6

共 15 条

[1]

DAVENPORT R, 1980, BIOL ACTIVITIES YEAS, P1

[2]

Dennis C., 1980, BIOL ACTIVITIES YEAS, P123

[3]

DICKINSON CH, 1976, MICROBIOLOGY AERIAL, P275

[4]

Enjalric F., 1988, Acta Horticulturae, P57

[5]

Hurley R, 1980, Soc Appl Bacteriol Symp Ser, V9, P231

[6]

J. C. GENTLES, 1969, The yeasts. Vol. I. Biology of yeasts., P107

[7]

LEGGAT I, 1988, ACTA HORTIC, V225, P93

[8] BACTERIAL CONTAMINANTS OF MICROPROPAGATED PLANT CULTURES [J].

LEIFERT, C ;

WAITES, WM ;

NICHOLAS, JR .

JOURNAL OF APPLIED BACTERIOLOGY, 1989, 67 (04) :353-361

[9] LACTOBACILLUS-PLANTARUM - A DELETERIOUS CONTAMINANT OF PLANT-TISSUE CULTURES [J].

LEIFERT, C ;

WAITES, WM ;

CAMOTTA, H ;

NICHOLAS, JR .

JOURNAL OF APPLIED BACTERIOLOGY, 1989, 67 (04) :363-370

[10] A REVISED MEDIUM FOR RAPID GROWTH AND BIO ASSAYS WITH TOBACCO TISSUE CULTURES [J].

MURASHIGE, T ;

SKOOG, F .

PHYSIOLOGIA PLANTARUM, 1962, 15 (03) :473-497

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