YEAST CONTAMINANTS OF MICROPROPAGATED PLANT CULTURES

被引：14

作者：

LEIFERT, C ^{[1
]}

WAITES, WM ^{[1
]}

NICHOLAS, JR ^{[1
]}

KEETLEY, JWW ^{[1
]}

机构：

[1] NEOPLANTS LTD, FRECKLETON PR4 1HU, LANCS, ENGLAND

来源：

JOURNAL OF APPLIED BACTERIOLOGY | 1990年 / 69卷 / 04期

关键词：

D O I：

10.1111/j.1365-2672.1990.tb01537.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Leifert, C., Waites, W.M., Nicholas, J.R. & Keetley, J.W. 1990. Yeast contaminants of micropropagated plant cultures. Journal of Applied Bacteriology69, 471–476. Of 36 yeast strains isolated from contaminated plant cultures 78% were Candida, 20%Rhodotorula and 2% were Cryptococcus species. Strains of Candida guilliermon‐dii represented 45% of all yeasts isolated. Yeasts grew rapidly on plant growth media with a pH of between 2.5 and 6.0 and decreased the medium pH to values of between 2.0 and 3.0. Candida yeasts growing on plant medium for 28 d metabolized about 75% of the sucrose and produced fermentation products such as ethanol and acetic acid. In contrast, Rhodotorula did not produce ethanol or acetic acid and only metabolized about 20% of the sucrose. Possible sources of contamination are discussed. Copyright © 1990, Wiley Blackwell. All rights reserved

引用

页码：471 / 476

页数：6