NEW MINI-TN5 DERIVATIVES FOR INSERTION MUTAGENESIS AND GENETIC-ENGINEERING IN GRAM-NEGATIVE BACTERIA

被引：72

作者：

ALEXEYEV, MF ^{[1
]}

SHOKOLENKO, IN ^{[1
]}

CROUGHAN, TP ^{[1
]}

机构：

[1] LOUISIANA STATE UNIV, CTR AGR, LOUISIANA AGR EXPT STN, RICE RES STN, CROWLEY, LA 70527 USA

来源：

CANADIAN JOURNAL OF MICROBIOLOGY | 1995年 / 41卷 / 11期

关键词：

R6K GAMMA-ORIGIN; SUICIDE VECTORS; RP4; ORIT; CHROMOSOMAL INSERTION;

D O I：

10.1139/m95-147

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Five mini-Tn5 derivatives encoding resistance to Km, Cm, Gm, Tc, and Sm, coupled with the polylinker of the pBluescriptII plasmid, were constructed. These derivatives are carried by an ampicillin-resistant plasmid that has a conditional origin of replication from plasmid R6K and origin of conjugal transfer from the broad host range plasmid RP4. The new vectors are smaller than those previously described and possess numerous unique restriction sites inside the minitransposons for gene cloning in addition to SfiI and NotI sites found in their predecessors.

引用

页码：1053 / 1055

页数：3

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