HIGHLY SENSITIVE FLUOROGENIC ENZYME-LINKED-IMMUNOSORBENT-ASSAY - DETECTION OF STAPHYLOCOCCAL ENTEROTOXIN-B

被引：13

作者：

BHATTI, AR

SIDDIQUI, YM

MICUSAN, VV

机构：

[1] DEI RES ESTAB SUFFIELD,RALSTON,AB,CANADA

[2] UNIV QUEBEC,INST ARMAND FRAPPIER,CTR RECH IMMUNOL,LAVAL H7N 4Z3,QUEBEC,CANADA

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 1994年 / 19卷 / 03期

关键词：

ENTEROTOXIN-B; FLUOROGENIC ELISA; MONOCLONAL ANTIBODY; STAPHYLOCOCCUS-AUREUS; TOXIN;

D O I：

10.1016/0167-7012(94)90068-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A highly sensitive, indirect double sandwich fluorogenic enzyme-linked immunosorbent assay (FELISA), has been developed for the detection of staphylococcal enterotoxin B (SEB). The described FELISA allows a SEB quantification of 0.1 fg/ml for purified toxin and a detection limit of at least 10 pg/g of contaminated food. Given the sensitivity of FELISA elaborate procedures for the extraction and concentration of enterotoxin from food samples became unnecessary. The monoclonal anti-SEB antibody (MCA a-SEB) used in the described method reacted with SEB only. The FELISA is simple to perform and, provided the reaction plates are pre-prepared, the results can be obtained in approximately 3 h.

引用

页码：179 / 187

页数：9

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