PURIFICATION AND BIOCHEMICAL-CHARACTERIZATION OF CHICKEN ANEMIA AGENT

被引：142

作者：

TODD, D ^{[1
]}

CREELAN, JL ^{[1
]}

MACKIE, DP ^{[1
]}

RIXON, F ^{[1
]}

MCNULTY, MS ^{[1
]}

机构：

[1] INST VIROL,GLASGOW G11 5JR,SCOTLAND

来源：

JOURNAL OF GENERAL VIROLOGY | 1990年 / 71卷

关键词：

D O I：

10.1099/0022-1317-71-4-819

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Chicken anaemia agent (CAA) was purified using differential centrifugation and successive cycles of equilibrium density gradient centrifugation using sucrose and CsCl. The purification method was dependent on the use of an antigen-detecting ELISA based on a CAA-specific monoclonal antibody. Virus particles banded at a density of 1.33 to 1.34 g/ml in CsCl and measured 23.5 ± 0.8 nm in diameter. Purified preparations contained one major polypeptide (M(r) 50000) and a single-stranded, circular DNA (2.3 kb). CAA shares some of the biochemical characteristics possessed by porcine circovirus and the virus associated with psittacine beak and feather disease.

引用

页码：819 / 823

页数：5

共 15 条

[11] A VERY SMALL PORCINE VIRUS WITH CIRCULAR SINGLE-STRANDED-DNA [J].