PURIFICATION AND AMINO-ACID-SEQUENCE OF HUMAN MOTILIN ISOLATED FROM A MOTILIN CONTAINING LIVER METASTASIS

被引：13

作者：

DECLERCQ, P

VANDERMEERS, A

VANASSCHE, G

FIASSE, R

DEPOORTERE, I

VANDERMEERSPIRET, MC

DEPREZ, P

PEETERS, T

机构：

[1] CATHOLIC UNIV LEUVEN, GUT HORMONE LAB, B-3000 LOUVAIN, BELGIUM

[2] FREE UNIV BRUSSELS, CHIM BIOL LAB, B-1070 BRUSSELS, BELGIUM

[3] UNIV CATHOLIQUE LOUVAIN, GASTROENTEROL UNIT, B-1200 BRUSSELS, BELGIUM

来源：

REGULATORY PEPTIDES | 1995年 / 55卷 / 01期

关键词：

MOTILIN; MOTILINOMA;

D O I：

10.1016/0167-0115(94)00094-E

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

The acid extract of a liver metastasis from a patient with elevated plasma motilin levels contained large quantities of motilin (3.37 mu g/ml). The extract was concentrated on a C-18-column and motilin was isolated by gel chromatography (Sephadex G-50) followed by cation ion exchange chromatography (RR5/5 Mono-S) and three successive steps of reverse phase chromatography (Nucleosil 300-5 C-18). The pure peptide was sequenced and the identity of porcine and human motilin was confirmed. This is the first report of a tumor containing large amounts of motilin.

引用

页码：79 / 84

页数：6

共 24 条

[1] THE ISOLATION AND CHARACTERIZATION OF RABBIT MOTILIN PRECURSOR CDNA [J].