THE CONSTRUCTION OF BACILLUS-THURINGIENSIS STRAINS EXPRESSING NOVEL ENTOMOCIDAL DELTA-ENDOTOXIN COMBINATIONS

被引：31

作者：

CRICKMORE, N ^{[1
]}

NICHOLLS, C ^{[1
]}

EARP, DJ ^{[1
]}

HODGMAN, TC ^{[1
]}

ELLAR, DJ ^{[1
]}

机构：

[1] USDA ARS,CTR PLANT GENE EXPRESS,ALBANY,CA 94710

来源：

BIOCHEMICAL JOURNAL | 1990年 / 270卷 / 01期

关键词：

D O I：

10.1042/bj2700133

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Using our recently reported method of electroporation to transform Bacillus thuringiensis [Bone and Ellar (1989) FEMS Microbiol. Lett. 58, 171-178], cloned B. thuringiensis entomocidal δ-endotoxin genes have been introduced into several native B. thuringiensis strains. In many cases the resulting transformants expressed both their native toxins and the cloned toxin, producing strains with broader toxicity spectra. The introduction of the var. tenebrionis toxin gene into B. thuringiensis var. israelensis resulted in a strain with activity against Pieris brassicae (cabbage white butterfly), an activity which neither parent strain possesses. We discuss further the possibility of synergism and also the problems associated with introducing cloned DNA by this method.

引用

页码：133 / 136

页数：4

共 49 条

[31]

Maniatis T., 1982, MOL CLONING

[32] TRANSFORMATION OF BACILLUS-THURINGIENSIS PROTOPLASTS BY PLASMID DEOXYRIBONUCLEIC-ACID [J].