SUBCELLULAR-LOCALIZATION OF POLYAMINES IN EMBRYOGENIC CALLUS OF WHITE SPRUCE (PICEA-GLAUCA)

被引：4

作者：

AMARASINGHE, V

CARLSON, JE

机构：

[1] UNIV BRITISH COLUMBIA,BIOTECHNOL LAB,VANCOUVER V6T 1Z3,BC,CANADA

[2] UNIV BRITISH COLUMBIA,DEPT FOOD SCI,VANCOUVER V6T 1Z4,BC,CANADA

来源：

CANADIAN JOURNAL OF BOTANY-REVUE CANADIENNE DE BOTANIQUE | 1994年 / 72卷 / 06期

关键词：

POLYAMINES; CYTOCHEMISTRY; IMMUNOLOCALIZATION; WHITE SPRUCE;

D O I：

10.1139/b94-100

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Polyamines were localized in embryogenic callus cells of Picea glauca by the fluorescent dye o-phthalaldehyde and polyclonal antibodies raised against putrescine, spermidine, and spermine. Localization by both methods showed higher levels of polyamines in the nuclei and nucleoli than in the cytoplasm. In contrast with previous reports on cytochemical localization of polyamines in animal cells, stain was always excluded from the condensed chromatin of spruce somatic embryo cells.

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收藏

页码：788 / 793

页数：6

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