A RETROGRADE LABELING TECHNIQUE FOR THE FUNCTIONAL-STUDY OF AIRWAY-SPECIFIC VISCERAL AFFERENT NEURONS

被引:45
作者
CHRISTIAN, EP [1 ]
TOGO, JA [1 ]
NAPER, KE [1 ]
KOSCHORKE, G [1 ]
TAYLOR, GA [1 ]
WEINREICH, D [1 ]
机构
[1] UNIV MARYLAND,SCH MED,DEPT PHARMACOL & EXPTL THERAPEUT,BALTIMORE,MD 21201
关键词
NODOSE GANGLION; DISSOCIATED NEURON; AFFERENT NEURON; C-FIBER; MICROELECTRODE; FLUORESCENT DYE; VITAL DYE; AIRWAY;
D O I
10.1016/0165-0270(93)90031-L
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The development of a method is described whereby primary afferent neurons that specifically innervate the airways in the guinea pig can be retrogradely labeled, acutely dissociated and studied functionally with electrophysiological techniques. Following administration of either dextran-tetramethylrhodamine, Fast Blue, or Fluorogold dye into the tracheal lumen, dye-labeled neurons can be visualized in 100-mum serial nodose ganglion sections. Control experiments show that labeling does not result from the undesirable spread of the dyes to target innervation fields in the gastrointestinal (GI) or cardiovascular (CV) systems. Neuronal somata retain dye label when acutely dissociated. Microelectrode studies provide evidence that the presence of the Rhodamine dye label and its fluorescent excitation neither alter basic electrophysiological membrane parameters nor the chemoreceptive properties of isolated neurons. Thus this new method will allow the isolation of individual airway-specific primary visceral afferent neurons for functional studies with multidisciplinary techniques.
引用
收藏
页码:147 / 160
页数:14
相关论文
共 30 条
[11]   CHEMORECEPTORS FOR SEROTONIN (5-HT), ACETYLCHOLINE (ACH), BRADYKININ (BK), HISTAMINE (H) AND GAMMA-AMINOBUTYRIC ACID (GABA) ON RABBIT VISCERAL AFFERENT NEURONS [J].
HIGASHI, H ;
UEDA, N ;
NISHI, S ;
GALLAGHER, JP ;
SHINNICKGALLAGHER, P .
BRAIN RESEARCH BULLETIN, 1982, 8 (01) :23-32
[12]   DIL AND DIO - VERSATILE FLUORESCENT DYES FOR NEURONAL LABELING AND PATHWAY TRACING [J].
HONIG, MG ;
HUME, RI .
TRENDS IN NEUROSCIENCES, 1989, 12 (09) :333-&
[13]   ELECTRICAL-PROPERTIES OF ACUTELY ISOLATED, IDENTIFIED RAT SPINAL DORSAL HORN PROJECTION NEURONS [J].
HUANG, LYM .
NEUROSCIENCE LETTERS, 1987, 82 (03) :267-272
[14]   ANALYSIS OF PASSIVE AND ACTIVE ELECTROPHYSIOLOGIC PROPERTIES OF NEURONS IN MAMMALIAN NODOSE GANGLIA MAINTAINED INVITRO [J].
JAFFE, RA ;
SAMPSON, SR .
JOURNAL OF NEUROPHYSIOLOGY, 1976, 39 (04) :802-815
[15]   BRAIN-STEM PROJECTIONS OF SENSORY AND MOTOR COMPONENTS OF THE VAGUS COMPLEX IN THE CAT .2. LARYNGEAL, TRACHEO-BRONCHIAL, PULMONARY, CARDIAC, AND GASTROINTESTINAL BRANCHES [J].
KALIA, M ;
MESULAM, MM .
JOURNAL OF COMPARATIVE NEUROLOGY, 1980, 193 (02) :467-508
[16]   FLUORESCENT LATEX MICROSPHERES AS A RETROGRADE NEURONAL MARKER FOR INVIVO AND INVITRO STUDIES OF VISUAL-CORTEX [J].
KATZ, LC ;
BURKHALTER, A ;
DREYER, WJ .
NATURE, 1984, 310 (5977) :498-500
[17]   PULMONARY AFFERENT ACTIVITY RECORDED FROM SYMPATHETIC-NERVES [J].
KOSTREVA, DR ;
ZUPERKU, EJ ;
HESS, GL ;
COON, RL ;
KAMPINE, JP .
JOURNAL OF APPLIED PHYSIOLOGY, 1975, 39 (01) :37-40
[18]  
Kuypers H.G.J.M., 1984, ADV CELL NEUROBIOL, V5, P307
[19]   LABELING AND RECORDING FROM DISSOCIATED TARGET-SPECIFIC RAT SUPERIOR CERVICAL-GANGLION NEURONS [J].
LUEBKE, JI ;
WEIGHT, FF ;
AGUAYO, LG .
NEUROSCIENCE LETTERS, 1992, 135 (02) :210-214
[20]  
LUNDBERG JM, 1984, CELL TISSUE RES, V235, P251