COMPARISON AT THE MICROSCOPIC SCALE OF MIXED FATTY-ACID PROTEIN LANGMUIR-BLODGETT-FILMS RESULTING FROM HORIZONTAL OR VERTICAL TRANSFER

Mixed Langmuir films in which behenic acid and glucose oxidase are associated have been transferred by vertical or horizontal lifting methods onto pyrolytic graphite. The resulting films have been compared by scanning electron microscopy and by scanning force microscopy. The structures observed at the microscopic scale are different for the two transfer techniques in terms of homogeneity, dimensions and orientations. At a nanoscopic scale however, the organization of enzyme molecules is quite analogous in both cases.