TRANSCRIPTION OF THE VIBRIO-CHOLERAE HEMOLYSIN GENE, HLYA, AND CLONING OF A POSITIVE REGULATORY LOCUS, HLYU

被引：36

作者：

WILLIAMS, SG

MANNING, PA

机构：

[1] Department of Microbiology and Immunology, University of Adelaide, Adelaide, South Australia, 5001

来源：

MOLECULAR MICROBIOLOGY | 1991年 / 5卷 / 08期

关键词：

D O I：

10.1111/j.1365-2958.1991.tb00825.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Transcription of the Vibrio cholerae hlyA gene, which encodes a cytotoxic haemolysin, has been investigated. The hlyA transcript initiates 430 nucleotides (nt) upstream of the translational start site. hlyA-cat transcriptional fusion constructs were active in V. cholerae but not in Escherichia coli. An hlyA-cat fusion was used to select, from a V. cholerae 017 plasmid library, a clone that could activate the hlyA promoter in E. coli. This regulatory locus has been designated hlyU. hlyU appears to be distinct from the previously described hlyR locus.

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页码：2031 / 2038

页数：8

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