KINETIC-ANALYSIS OF SOY-PROTEIN DENATURATION BY A TEMPERATURE-PROGRAMMED HEAT-DENATURATION TECHNIQUE

被引:8
作者
YOSHII, H [1 ]
FURUTA, T [1 ]
NOMA, S [1 ]
NODA, T [1 ]
机构
[1] AJINOMOTO CO INC,KAWASAKI,KANAGAWA 210,JAPAN
来源
AGRICULTURAL AND BIOLOGICAL CHEMISTRY | 1990年 / 54卷 / 04期
关键词
D O I
10.1080/00021369.1990.10870067
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
The soy-protein denaturation rates of defatted soy flour and isolated soy protein of varying moisture content were analyzed by a temperature-programmed heat-denaturation (TPHD) technique with a hand-press reactor. The degree of protein denaturation of the soy proteins, which is defined as the solubility in 0.1, m, phosphate buffer (pH 8.0) containing 1% SDS and 1% 2-ME, could be estimated with the TPHD model as a first-order heat-denaturation process. A kinetic compensation effect was observed for the protein denaturation of both soy proteins, the activation energy of the denaturation rate decreasing linearly with a moisture content below 0.4. © 1990 by the Japan Society for Bioscience, Biotechnology, and Agrochemistry. All rights reserved.
引用
收藏
页码:863 / 869
页数:7
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