LANGERHANS CELLS IN HUMAN ALLERGIC CONTACT-DERMATITIS CONTAIN VARYING NUMBERS OF BIRBECK GRANULES - DOUBLE STAINING IMMUNOHISTOCHEMISTRY WITH OKT6 AND LAG ANTIBODY

Dynamic changes in human Langerhans cells (LCs) were studied with OK T6, anti-HLA-DR antibody, and Lag antibody in allergic contact dermatitis (ACD). Both T6-positive (T6+) cells and Lag-positive (Lag+) cells in the epidermis decreased in number from 0 to 48 h, but then gradually increased after day 7 of ACD. Lag+ cells after day 7 manifested a variety of staining intensities from weak to strong. It was also shown, after day 7, that some T6+ cells were Lag negative whereas all Lag+ cells were T6 positive. Flow cytometric analysis suggested that Lag-strongly-positive cells and Lag-weakly-positive cells belonged to the same population, and that the relative amount of Lag antigens in T6+ LCs gradually increased after day 7. Immunoelectron microscopy revealed that the Lag-strongly-positive cells contained numerous Lag-reactive Birbeck granules (BGs) whereas the Lag-weakly-positive cells contained fewer BGs in the cytoplasm. In some Lag-weakly-positive cells, no BGs were detected. © 1990 Springer-Verlag.