NOVEL PROTEINS MEDIATE AN INTERACTION BETWEEN CLATHRIN-COATED VESICLES AND POLYMERIZING ACTIN-FILAMENTS

被引：12

作者：

KOHTZ, DS ^{[1
]}

HANSON, V ^{[1
]}

PUSZKIN, S ^{[1
]}

机构：

[1] CUNY MT SINAI SCH MED,DEPT PATHOL,MOLEC & CELLULAR PATHOL LABS,NEW YORK,NY 10029

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1990年 / 192卷 / 02期

关键词：

D O I：

10.1111/j.1432-1033.1990.tb19226.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A monoclonal antibody, A‐7C11, was generated which reacts with two polypeptides of 40 kDa and 80 kDa associated with the coat proteins of purified brain clathirn‐coated vesicles. The 40‐kDa antigen was purified and found to display actin‐binding properties. Negative‐staining electron microscopy showed that one of the antigens reactive with A‐7C11 appears to mediate the association of isolated clathrin‐coated vesicles with assembling actin filaments in vitro. Immunofluorescence microscopy of cultured fibroblasts with A‐7C11 revealed the antigens aligned with both actin filaments and as punctate structures near the plasma membrane. The data suggest that the interaction between clathrin‐coated vesicles and the actin cytoskeleton is mediated by antigens identified by monoclonal antibody A‐7C11. Copyright © 1990, Wiley Blackwell. All rights reserved

引用

页码：291 / 298

页数：8

共 36 条

[1] Role of the coated endocytic vesicle in the uptake of receptor-bound low density lipoprotein in human fibroblasts [J].

ANDERSON, RGW ;

BROWN, MS ;

GOLDSTEIN, JL .

CELL, 1977, 10 (03) :351-364

[2] ACTOMYOSIN-LIKE PROTEIN IN BRAIN [J].

BERL, S ;

PUSZKIN, S ;

NICKLAS, WJ .

SCIENCE, 1973, 179 (4072) :441-446

[3]

BLOOM W, 1980, P NATL ACAD SCI USA, V7, P5520

[4] PEPTIDE ANTIBODY SPECIFIC FOR THE AMINO TERMINUS OF SKELETAL-MUSCLE ALPHA-ACTIN [J].

BULINSKI, JC ;

KUMAR, S ;

TITANI, K ;

HAUSCHKA, SD .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1983, 80 (06) :1506-1510

[5]

FINE RE, 1984, INT REV CYTOL, V91, P1

[6] INVOLVEMENT OF VESICLE COAT MATERIAL IN CASEIN SECRETION AND SURFACE REGENERATION [J].

FRANKE, WW ;

LUDER, MR ;

KARTENBECK, J ;

ZERBAN, H ;

KEENAN, TW .

JOURNAL OF CELL BIOLOGY, 1976, 69 (01) :173-195

[7] EXIT OF NEWLY SYNTHESIZED MEMBRANE-PROTEINS FROM THE TRANS CISTERNA OF THE GOLGI-COMPLEX TO THE PLASMA-MEMBRANE [J].

GRIFFITHS, G ;

PFEIFFER, S ;

SIMONS, K ;

MATLIN, K .

JOURNAL OF CELL BIOLOGY, 1985, 101 (03) :949-964

[8] NOVEL REGULATORY ROLE OF PHOSPHORYLATED CLATHRIN LIGHT CHAIN-BETA IN BOVINE BRAIN COATED VESICLES [J].

HANSON, VG ;

SCHOOK, WJ ;

PUSZKIN, S .

JOURNAL OF NEUROCHEMISTRY, 1990, 54 (01) :46-50

[9] EVIDENCE FOR RECYCLING OF SYNAPTIC VESICLE MEMBRANE DURING TRANSMITTER RELEASE AT FROG NEUROMUSCULAR JUNCTION [J].

HEUSER, JE ;

REESE, TS .

JOURNAL OF CELL BIOLOGY, 1973, 57 (02) :315-344

[10] COMPARISON OF THE PRIMARY STRUCTURES OF CLATHRIN LIGHT-CHAINS FROM BOVINE BRAIN AND ADRENAL-GLAND BY PEPTIDE-MAPPING [J].

HOLMES, N ;

BIERMANN, JS ;

BRODSKY, FM ;

BHARUCHA, D ;

PARHAM, P .

EMBO JOURNAL, 1984, 3 (07) :1621-1627

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