ANALYSIS OF A GENOMIC DNA SEGMENT CARRYING THE WHEAT HIGH-MOLECULAR-WEIGHT (HMW) GLUTENIN BX17 SUBUNIT AND ITS USE AS AN RFLP MARKER

被引：88

作者：

REDDY, P ^{[1
]}

APPELS, R ^{[1
]}

机构：

[1] CSIRO,DIV PLANT IND,WHEAT RES UNIT,POB 7,N RYDE,NSW 2113,AUSTRALIA

来源：

THEORETICAL AND APPLIED GENETICS | 1993年 / 85卷 / 05期

关键词：

WHEAT; GLUTENIN; DOUGH; EVOLUTION; RFLPS;

D O I：

10.1007/BF00220921

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

A genomic fragment containing the Bx17 high-molecular-weight (HMW) glutenin gene was isolated from a wheat genomic library. The fragment contains a coding region of 2.82 kb with 1.98-kb downstream and 12.8-kb upstream flanking regions. The fragment was sequenced and compared with previously published glutenin genes from chromosomes 1A, 1B and 1D using a computer alignment package. The Bx17 gene shows marked similarity to the Bx7 gene sequence. A phenetic tree derived from the alignments is presented. Also shown are restriction fragment length polymorphisms (RFLPs) at the glutenin loci in a set of Australian and international wheat varieties using different regions of the glutenin clone as probes. The RFLPs correlated well with the protein composition in all cultivars analysed.

引用

页码：616 / 624

页数：9