MOLECULAR-CLONING AND TISSUE-SPECIFIC EXPRESSION OF FRUCTOSE 6-PHOSPHATE,2-KINASE-FRUCTOSE 2,6-BISPHOSPHATASE OF RAT-BRAIN

被引：28

作者：

WATANABE, F

SAKAI, A

FURUYA, E

UYEDA, K

机构：

[1] OSAKA MED COLL,DEPT CHEM,TAKATSUKI,OSAKA 569,JAPAN

[2] UNIV TEXAS,SW MED CTR,DALLAS,TX 75216

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1994年 / 198卷 / 01期

关键词：

D O I：

10.1006/bbrc.1994.1047

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A cDNA clone (3591 base pairs) encoding an isozyme of Fructose 6-phosphate, 2-kinase:Fructose 2,6-bisphosphatase was isolated from a rat brain cDNA library. The 5′ sequence of this clone (1241 base pairs) was identical to that of the heart type isozyme cDNA (except for 7 base pair mismatches), but the 3′ nucleotide sequence (2024 base pairs) was completely different. Its deduced amino acid sequence showed that the enzyme lacked a regulatory domain which contained phosphorylation sites for protein kinase A and C. The results of Northern blotting and polymerase chain reaction demonstrated that the mRNA, 7.4 kilobases long, was expressed also in heart, testis, liver, and skeletal muscle. © 1994 Academic Press, Inc.

引用

页码：335 / 340

页数：6

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