FLOW-INJECTION SOLID-PHASE CHEMILUMINESCENT IMMUNOASSAY USING A MEMBRANE-BASED REACTOR

被引：43

作者：

LIU, HJ ^{[1
]}

YU, JC ^{[1
]}

BINDRA, DS ^{[1
]}

GIVENS, RS ^{[1
]}

WILSON, GS ^{[1
]}

机构：

[1] UNIV KANSAS,CTR BIOANALYT RES,LAWRENCE,KS 66045

来源：

ANALYTICAL CHEMISTRY | 1991年 / 63卷 / 07期

关键词：

D O I：

10.1021/ac00007a004

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A flow injection analysis (FIA) sandwich immunoassay (ELISA) using a polyvinylidene difluoride polymer based membrane immunosorbent has been proposed. The membrane to which the antigen (bovine IgG) is attached is mounted in a flow cell (5-mu-L dead volume). Analyte in the form of mouse antibovine IgG is injected into the flowing stream followed by a goat anti-mouse IgG horseradish peroxidase (HRP) conjugate. The HRP was used to catalyze the enhanced luminol reaction, resulting in chemiluminescence, which was detected directly within the immunoreactor. A detection limit of 1 fmol was obtained while spiked serum samples gave precision of 8.7%.

引用

页码：666 / 669

页数：4

共 20 条

[1] RAPID HETEROGENEOUS COMPETITIVE ELECTROCHEMICAL IMMUNOASSAY FOR IGG IN THE PICOMOLE RANGE [J].