COMPARISON BETWEEN ORIENTATIONAL AND CONFORMATIONAL ORDERS IN FLUID LIPID BILAYERS

The orientational order as determined by H-2 NMR and the infrared frequencies of the C-H stretching modes of the methylene groups have been measured for several systems (POPC, POPC/cholesterol and POPE), all in the fluid phase, and then were compared; this work reveals an unexpected linear correlation between them. This experimental result shows that both measurements are essentially sensitive to a common motion, most likely trans/gauche isomerisation. This new correlation with those already found in the literature suggest that several measurements related tc the hydrophobic core of the fluid bilayer describe different aspects of a universal behavior. The correlation presented here does not extend to the lipid in gel phase where slower motions affect the NMR lineshape.