MAST-CELL TRYPTASE LEVELS IN NORMAL CANINE TISSUES

被引：13

作者：

MYLES, AD ^{[1
]}

HALLIWELL, REW ^{[1
]}

BALLAUF, B ^{[1
]}

MILLER, HRP ^{[1
]}

机构：

[1] UNIV EDINBURGH,ROYAL DICK SCH VET STUDIES,DEPT VET CLIN STUDIES,EDINBURGH EH9 1QH,MIDLOTHIAN,SCOTLAND

来源：

VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY | 1995年 / 46卷 / 3-4期

基金：

英国惠康基金;

关键词：

D O I：

10.1016/0165-2427(94)05358-Y

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Levels of canine tryptase from various tissues were quantified using a competition enzyme-linked immunosorbent assay (ELISA). The assay utilises an affinity-purified rabbit anti-tryptase antibody in the solid phase and alkaline-phosphatase conjugated tryptase together with unlabelled tryptase in the fluid phase. The assay will rapidly quantify 40-5000 ng ml(-1) of tryptase in tissue extracts. Tissues from the skin, gut, liver and lung were studied, of which canine gut appeared to contain the highest levels of tryptase per milligram wet weight, which may suggest an important role for this enzyme at this site. This assay may prove valuable in assessing the role of mast cells in various disease states in the dog.

引用

页码：223 / 235

页数：13