VECTORS TO FACILITATE THE CREATION OF TRANSLATIONAL FUSIONS TO THE MALTOSE-BINDING PROTEIN OF ESCHERICHIA-COLI

被引：3

作者：

AITKEN, R

GILCHRIST, J

SINCLAIR, MC

机构：

[1] Department of Microbiology, University of Glasgow, Glasgow

来源：

GENE | 1994年 / 144卷 / 01期

关键词：

EXPRESSION; MBP; STAPHYLOCOCCAL ENTEROTOXIN A;

D O I：

10.1016/0378-1119(94)90205-4

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A set of vectors has been constructed to facilitate the fusion of heterologous sequences to the C terminus of the maltose-binding protein (MBP) of Escherichia coli. The plasmids carry a cloning region comprising two blunt cloning sites, a BamHI site and multiple stop codons, and this has been placed in each reading frame so that translational fusions to MBP can be generated and manipulated with ease. To demonstrate the utility of this system, recombinant proteins have been engineered in which staphylococcal enterotoxin A has been fused to MBP.

引用

页码：69 / 73

页数：5

共 23 条

[1] DEVELOPMENT OF AN IMMUNOASSAY USING RECOMBINANT MALTOSE-BINDING PROTEIN STA FUSIONS FOR QUANTITATING ANTIBODY-RESPONSES AGAINST STA, THE HEAT-STABLE ENTEROTOXIN OF ESCHERICHIA-COLI [J].