MICROASSAY FOR THE SIMULTANEOUS DETERMINATION OF COCAINE, NORCOCAINE, BENZOYLECGONINE AND BENZOYLNORLECGONINE BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：34

作者：

SANDBERG, JA ^{[1
]}

OLSEN, GD ^{[1
]}

机构：

[1] OREGON HLTH SCI UNIV,SCH MED,DEPT PHARMACOL,L221,3181 SW SAM JACKSON PK RD,PORTLAND,OR 97201

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1990年 / 525卷 / 01期

关键词：

D O I：

10.1016/S0378-4347(00)83384-8

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An improved method for the simultaneous determination of cocaine, norcocaine, benzoylecgonine and benzoylnorecgonine using reversed-phase high-performance liquid chromatography with ultraviolet detection is described. Following solid-phase extraction, chromatography was performed using a column containing an octadecylsilica-coated packing, eluted with 6% acetonitrile in phosphate buffer, pH 2.1, and detected at 233 nm. Using 80-μl samples, the detection limit is 18 ng/ml for benzoylecgonine and benzoylenorecgonine and 35 ng/ml for cocaine and norcocaine. The coefficients of variation range from 3.5% (benzoylecgonine) to 7.0% (norcocaine). The procedure has been applied to samples of guinea pig plasma, urine and amniotic fluid and human urine. © 1990.

引用

页码：113 / 121

页数：9

共 18 条

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