QUANTITATION OF L-EPINEPHRINE AND DETERMINATION OF THE D-EPINEPHRINE L-EPINEPHRINE ENANTIOMER RATIO IN A PHARMACEUTICAL FORMULATION BY CAPILLARY ELECTROPHORESIS

A procedure for the quantitation of l-epinephrine and the determination of the d-/l-epinephrine ratio in a pharmaceutical formulation containing l-epinephrine is described. The optical isomers of epinephrine were resolved by capillary electrophoresis with a buffer containing heptakis-(2,6-di-o-methyl)-beta-cyclodextrin. Quantitation was achieved with the use of an internal standard, 1-pseudoephedrine. Results with and without internal standard correction illustrate the improved reproducibility possible with an internal standard.