DETECTION OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 GENOMIC RNA IN PLASMA SAMPLES BY REVERSE-TRANSCRIPTION POLYMERASE CHAIN-REACTION

被引：32

作者：

BAGNARELLI, P

MENZO, S

MANZIN, A

GIACCA, M

VARALDO, PE

CLEMENTI, M

机构：

[1] UNIV ANCONA,SCH MED,INST MICROBIOL,I-60100 ANCONA,ITALY

[2] UNIV TRIESTE,INT CTR GENET ENGN & BIOTECHNOL,TRIESTE,ITALY

[3] UNIV TRIESTE,INST MICROBIOL,TRIESTE,ITALY

来源：

JOURNAL OF MEDICAL VIROLOGY | 1991年 / 34卷 / 02期

关键词：

POLYMERASE CHAIN REACTION; HUMAN IMMUNODEFICIENCY VIRUS TYPE-1; VIREMIA;

D O I：

10.1002/jmv.1890340204

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

An application of the polymerase chain reaction (PCR) to the direct detection of human immunodeficiency virus type 1 (HIV-1) viremia is described. The amplification of specific HIV-1 sequences of gag and env viral genes was carried out after the reverse-transcription of plasma samples (plasma RT-PCR) from seropositive subjects. The assay is faster and cheaper than detection of specific HIV-1 transcripts from peripheral blood mononuclear cells by RT-PCR. The data suggest that HIV-1 viremia is detectable by plasma RT-PCR in a large proportion of seropositive individuals.

引用

收藏

页码：89 / 95

页数：7

相关论文

共 54 条

[1] ISOLATION OF HUMAN-IMMUNODEFICIENCY-VIRUS (HIV) FROM PLASMA DURING PRIMARY HIV-INFECTION [J].

ALBERT, J ;

GAINES, H ;

SONNERBORG, A ;

NYSTROM, G ;

PEHRSON, PO ;

CHIODI, F ;

VONSYDOW, M ;

MOBERG, L ;

LIDMAN, K ;

CHRISTENSSON, B ;

ASJO, B ;

FENYO, EM .

JOURNAL OF MEDICAL VIROLOGY, 1987, 23 (01) :67-73

[2]

BAGNARELLI P, 1991, IN PRESS J VIROLOGIC

[3]

BALTIMORE D, 1990, NEW ENGL J MED, V322, P1468

[4] ISOLATION OF A T-LYMPHOTROPIC RETROVIRUS FROM A PATIENT AT RISK FOR ACQUIRED IMMUNE-DEFICIENCY SYNDROME (AIDS) [J].

BARRESINOUSSI, F ;

CHERMANN, JC ;

REY, F ;

NUGEYRE, MT ;

CHAMARET, S ;

GRUEST, J ;

DAUGUET, C ;

AXLERBLIN, C ;

VEZINETBRUN, F ;

ROUZIOUX, C ;

ROZENBAUM, W ;

MONTAGNIER, L .

SCIENCE, 1983, 220 (4599) :868-871

[5] CYTOMEGALOVIRUS ACTIVATES TRANSCRIPTION DIRECTED BY THE LONG TERMINAL REPEAT OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 [J].

BARRY, PA ;

PRATTLOWE, E ;

PETERLIN, BM ;

LUCIW, PA .

JOURNAL OF VIROLOGY, 1990, 64 (06) :2932-2940

[6]

BEDNARIK DP, 1987, J VIROL, V173, P1253

[7] NUCLEOTIDE-SEQUENCE EVIDENCE FOR RELATIONSHIP OF AIDS RETROVIRUS TO LENTIVIRUSES [J].

CHIU, IM ;

YANIV, A ;

DAHLBERG, JE ;

GAZIT, A ;

SKUNTZ, SF ;

TRONICK, SR ;

AARONSON, SA .

NATURE, 1985, 317 (6035) :366-368

[8]

CHOMCZYNSKI P, 1987, ANAL BIOCHEM, V162, P156, DOI 10.1016/0003-2697(87)90021-2

[9] INVITRO ACTIVATION OF THE HIV-1 ENHANCER IN EXTRACTS FROM CELLS TREATED WITH A PHORBOL ESTER TUMOR PROMOTER [J].

DINTER, H ;

CHIU, R ;

IMAGAWA, M ;

KARIN, M ;

JONES, KA .

EMBO JOURNAL, 1987, 6 (13) :4067-4071

[10] TUMOR NECROSIS FACTOR A ACTIVATES HUMAN IMMUNODEFICIENCY VIRUS TYPE-1 THROUGH INDUCTION OF NUCLEAR FACTOR BINDING TO THE NF-KAPPA-B SITES IN THE LONG TERMINAL REPEAT [J].

DUH, EJ ;

MAURY, WJ ;

FOLKS, TM ;

FAUCI, AS ;

RABSON, AB .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (15) :5974-5978

← 1 2 3 4 5 6 →