DETECTION OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 GENOMIC RNA IN PLASMA SAMPLES BY REVERSE-TRANSCRIPTION POLYMERASE CHAIN-REACTION

被引：32

作者：

BAGNARELLI, P

MENZO, S

MANZIN, A

GIACCA, M

VARALDO, PE

CLEMENTI, M

机构：

[1] UNIV ANCONA,SCH MED,INST MICROBIOL,I-60100 ANCONA,ITALY

[2] UNIV TRIESTE,INT CTR GENET ENGN & BIOTECHNOL,TRIESTE,ITALY

[3] UNIV TRIESTE,INST MICROBIOL,TRIESTE,ITALY

来源：

JOURNAL OF MEDICAL VIROLOGY | 1991年 / 34卷 / 02期

关键词：

POLYMERASE CHAIN REACTION; HUMAN IMMUNODEFICIENCY VIRUS TYPE-1; VIREMIA;

D O I：

10.1002/jmv.1890340204

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

An application of the polymerase chain reaction (PCR) to the direct detection of human immunodeficiency virus type 1 (HIV-1) viremia is described. The amplification of specific HIV-1 sequences of gag and env viral genes was carried out after the reverse-transcription of plasma samples (plasma RT-PCR) from seropositive subjects. The assay is faster and cheaper than detection of specific HIV-1 transcripts from peripheral blood mononuclear cells by RT-PCR. The data suggest that HIV-1 viremia is detectable by plasma RT-PCR in a large proportion of seropositive individuals.

引用

页码：89 / 95

页数：7

共 54 条

[11] EFFICIENT ISOLATION OF HIV FROM PLASMA DURING DIFFERENT STAGES OF HIV INFECTION [J].