A series of monoclonal antibodies were developed against bovine rotavirus Q17. Among the five high affinity antibodies characterized, two (RQ31 and RQ4) were able to neutralize type G 6 viruses and may be specific for PB1 type virus. Seventy seven feces from diarrheic calves were tested by ''double sandwich'' ELISA using four monoclonal and one polyclonal anti-rotavirus antibodies. The combination of mono- and polyclonal antibodies thus appears to be a more efficient strategy for detection and typing of bovine rotaviruses.