APOLIPOPROTEIN-B MESSENGER-RNA EDITING IN-VITRO IS A ZINC-DEPENDENT PROCESS

被引:27
作者
BARNES, C
SMITH, HC
机构
[1] UNIV ROCHESTER,DEPT BIOCHEM,ROCHESTER,NY 14642
[2] UNIV ROCHESTER,DEPT PATHOL,ROCHESTER,NY 14642
[3] UNIV ROCHESTER,CTR CANC,ROCHESTER,NY 14642
关键词
D O I
10.1006/bbrc.1993.2634
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Apolipoprotein B mRNA editing involves the conversion of a cytidine at nucleotide 6666 to a uridine, thereby creating a translational stop codon from a glutamine codon. Recent evidence suggest that a cytidine deaminase is responsible for catalyzing the C to U conversion. All known nucleotide deaminases require zinc as part of their catalytic domain. Utilizing the selective chelator for zinc, 1,10-penanthroline, we demonstrate that in vitro editing activity in rat liver extracts is zinc dependent. These data, taken together with recent reports, strongly support a role for a cytidine deaminase and nucleotide conversion as the catalytic mechanism for apoB mRNA editing. © 1993 Academic Press, Inc.
引用
收藏
页码:1410 / 1414
页数:5
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