MICROPROPAGATION OF GLORIOSA - TOWARDS A PRACTICAL PROTOCOL

被引：17

作者：

CUSTERS, JBM

BERGERVOET, JHW

机构：

[1] DLO-Centre for Plant Breeding and Reproduction Research (CPRO-DLO), Wageningen

来源：

SCIENTIA HORTICULTURAE | 1994年 / 57卷 / 04期

关键词：

GLORIOSA; HYBRIDS; IN-VITRO GROWTH; MICROPROPAGATION;

D O I：

10.1016/0304-4238(94)90115-5

中图分类号：

S6 [园艺];

学科分类号：

0902 ;

摘要：

Sprouts from tubers or tips from tubers during the first month of storage proved to be good explants to start cultures of Gloriosa. Addition of a low level of 6-benzyladenine (BA) in the medium (1 mg l-1) improved in vitro plant growth, whereas a high level of BA (10 mg l-1) caused a proliferation of multiple shoots from the rhizome meristems. By applying alternately the high and low BA levels, a method of continued micropropagation was achieved, which resulted in a four- to seven-fold multiplication of qualitatively good plantlets every 18 weeks. Transplantation into soil was only possible after the plants had formed tubers.

引用

页码：323 / 334

页数：12