CASSETTE VECTORS DIRECTING EXPRESSION OF T-CELL RECEPTOR GENES IN TRANSGENIC MICE

被引：135

作者：

KOUSKOFF, V ^{[1
]}

SIGNORELLI, K ^{[1
]}

BENOIST, C ^{[1
]}

MATHIS, D ^{[1
]}

机构：

[1] ULP, INST GENET & BIOL MOLEC & CELLULAIRE, CNRS, INSERM, F-67404 ILLKIRCH GRAFFENSTADEN, FRANCE

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1995年 / 180卷 / 02期

关键词：

T CELL RECEPTOR; TRANSGENIC MOUSE; CASSETTE VECTOR;

D O I：

10.1016/0022-1759(95)00002-R

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe a pair of cassette vectors that can be used to express rearranged T cell receptor genes in transgenic mice. Short DNA fragments containing rearranged V alpha and V beta segments are readily amplified from T cells and introduced between artificial cloning sites. Transgene-derived mRNAs are transcribed under the control of the natural TCR alpha and -beta promoter/enhancer elements. Using this vector, we have obtained transgenic mouse lines which display transgene-encoded TCR alpha and beta chains on a majority of T cells.

引用

页码：273 / 280

页数：8

共 17 条

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