A DERIVATIZATION PROCEDURE SUITABLE FOR HPLC ANALYSIS OF CLENBUTEROL

被引：14

作者：

BIONDI, PA

GUIDOTTI, L

MONTANA, M

MANCA, F

BRAMBILLA, G

LUCARELLI, C

机构：

[1] CTR ANTIDOPING JOCKEY CLUB ITALIANO,MILAN,ITALY

[2] IST SUPER SANITA,I-00161 ROME,ITALY

来源：

JOURNAL OF CHROMATOGRAPHIC SCIENCE | 1991年 / 29卷 / 05期

关键词：

D O I：

10.1093/chromsci/29.5.190

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A precolumn derivatization method has been set up for the analysis of clenbuterol by high-performance liquid chromatography (HPLC) with UV detection. After preliminary studies, N,N-dimethylaniline was chosen as reagent against clenbuterol diazonium salt to form a diazocompound. The final derivative was separated on a reversed-phase column using a phosphate buffer-acetonitrile mixture as eluent. The reproducibility was increased using a compound homologous to clenbuterol as the internal standard. The method was applied to the analysis of clenbuterol in calf urine.

引用

页码：190 / 193

页数：4

共 8 条

[1]

BRUNN H, 1988, FLEISCHWIRTSCHAFT, V68, P1476

[2] CLENBUTEROL RESIDUE ANALYSIS BY HPLC-HPTLC IN URINE AND ANIMAL-TISSUES [J].