USE OF CYCLODEXTRINS IN ENZYMOLOGY TO ENHANCE THE SOLUBILITY OF HYDROPHOBIC COMPOUNDS IN WATER

被引：17

作者：

OTERO, C

CRUZADO, C

BALLESTEROS, A

机构：

[1] Institute de Catalisis, C.S.I.C., Madrid

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 1991年 / 27卷 / 02期

关键词：

LIPASES; HYDROLASES; CYCLODEXTRINS; SOLUBILIZING AGENTS; CYCLODEXTRINS AS; INSOLUBLE SUBSTRATES; CYCLODEXTRINS AS SOLUBILIZERS OF;

D O I：

10.1007/BF02921526

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In enzyme catalysis, hydrophobic compounds are usually made soluble in water by the addition of low amounts of a water-miscible organic solvent. In the present study, taking advantage of the solubilizing properties of inclusion compounds (cyclodextrins), a new methodology is proposed. The hydrolysis of p-nitrophenyl esters catalyzed by several hydrolases (lipase from C. cylindracea, porcine pancreatic lipase, R. arrhizus lipase and cholesterol esterase) in the presence of cyclodextrins (CD), has been investigated. This procedure presents many advantages: 1. The enzyme parameters are not affected substantially (the catalytic constant may even increase); 2. The reproducibility is higher than with previous methods; 3. CDs enhance the solubility of substrates; and 4. CDs do not destabilize enzyme structure. In some cases a thermal stabilization is observed.

引用

页码：185 / 194

页数：10

共 27 条

[1] INFLUENCE OF ORGANIC AQUEOUS-MEDIA IN THE DNASE ACTIVITY OF MICROCOCCAL ENDONUCLEASE [J].