TRANSCRIPTIONAL REGULATION OF THE HUMAN H-FERRITIN-ENCODING GENE (FERH) IN G418-TREATED CELLS - ROLE OF THE B-BOX-BINDING FACTOR

被引：26

作者：

BEVILACQUA, MA ^{[1
]}

FANIELLO, MC ^{[1
]}

RUSSO, T ^{[1
]}

CIMINO, F ^{[1
]}

COSTANZO, F ^{[1
]}

机构：

[1] UNIV NAPLES,DIPARTIMENTO BIOCHIM & BIOTECNOL MED,I-80131 NAPLES,ITALY

来源：

GENE | 1994年 / 141卷 / 02期

关键词：

GENE CONTROL; RAT HOMOLOGIES; DNA BAND-SHIFT ASSAY; CHLORAMPHENICOL ACETYLTRANSFERASE;

D O I：

10.1016/0378-1119(94)90587-8

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We have analysed the molecular basis underlying the increase in ferritin heavy-chain mRNA (FERH) levels in cells exposed to the antibiotic Geneticin (G418). Transient transfection experiments demonstrate that this increase is paralleled by an enhanced transcription driven by the promoter (pFERH) for the human FERH gene, in which the most proximal promoter element (B-box) appears to play a key role. This region is conserved in human and rat, and binds an unknown factor. The DNA-protein complex composed of B-box-binding factor and its cis-element becomes more abundant in the G4l8-treated cells, as compared with the untreated ones.

引用

页码：287 / 291

页数：5

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