REPLICATION OF CHLORELLA VIRUS PBCV-1 AND HOST KARYOTYPE DETERMINATION STUDIED WITH PULSED-FIELD GEL-ELECTROPHORESIS

被引：6

作者：

MCCLUSKEY, K ^{[1
]}

GRAVES, MV ^{[1
]}

MILLS, D ^{[1
]}

MEINTS, RH ^{[1
]}

机构：

[1] OREGON STATE UNIV,DEPT BOT & PLANT PATHOL,CORVALLIS,OR 97331

来源：

JOURNAL OF PHYCOLOGY | 1992年 / 28卷 / 06期

关键词：

CHLORELLA; CHLOROPHYTA; CHROMOSOMES; DNA; KARYOTYPE; VIRUS;

D O I：

10.1111/j.0022-3646.1992.00846.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

We demonstrated the time-course of DNA replication of the 330-kbp Chlorella virus PBCV-1 by pulsed-field gel electrophoresis. Viral DNA replication began between 90 and 120 min postinfection in cell samples produced without first removing cell walls. The host nuclear genome was degraded beginning almost immediately after infection, but some host DNA remained even after 360 min postinfection. The karyotype of the host alga consisted of nine resolvable bands that we presume to represent 13 chromosomes based on band intensity. The chromosomes ranged in size from 1.1 to 6.5 megabase pairs (Mbp). A summation of the sizes of the bands predicts a genome size of 39-45 Mbp of DNA.

引用

页码：846 / 850

页数：5

共 28 条

[1] ELECTROPHORETIC KARYOTYPE OF ASPERGILLUS-NIDULANS [J].

BRODY, H ;

CARBON, J .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (16) :6260-6263

[2] AN ELECTROPHORETIC KARYOTYPE FOR YEAST [J].

CARLE, GF ;

OLSON, MV .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (11) :3756-3760

[3] PURIFICATION AND SOME PROPERTIES OF A DOUBLE-STRANDED DNA CONTAINING VIRUS-LIKE PARTICLE FROM URONEMA-GIGAS, A FILAMENTOUS EUKARYOTIC GREEN-ALGA [J].

COLE, A ;

DODDS, JA ;

HAMILTON, RI .

VIROLOGY, 1980, 100 (01) :166-174

[4] CHROMOSOME SIZE POLYMORPHISMS IN PLASMODIUM-FALCIPARUM CAN INVOLVE DELETIONS AND ARE FREQUENT IN NATURAL PARASITE POPULATIONS [J].

CORCORAN, LM ;

FORSYTH, KP ;

BIANCO, AE ;

BROWN, GV ;

KEMP, DJ .

CELL, 1986, 44 (01) :87-95

[5] IDENTIFICATION OF TEMPERATURE-SENSITIVE MUTANTS OF VACCINIA VIRUS THAT ARE DEFECTIVE IN CONVERSION OF CONCATEMERIC REPLICATIVE INTERMEDIATES TO THE MATURE LINEAR DNA GENOME [J].

DELANGE, AM .

JOURNAL OF VIROLOGY, 1989, 63 (06) :2437-2444

[6] RESTRICTION SITE MAP OF THE CHLORELLA VIRUS PBCV-1 GENOME [J].

GIRTON, LE ;

VANETTEN, JL .

PLANT MOLECULAR BIOLOGY, 1987, 9 (03) :247-257

[7]

Godward MBE, 1966, CHROMOSOMES ALGAE

[8]

HENRY EC, 1990, J PHYCOL, V28, P517

[9] ELECTROPHORETIC KARYOTYPING AND CHROMOSOMAL GENE-MAPPING OF CHLORELLA [J].

HIGASHIYAMA, T ;

YAMADA, T .

NUCLEIC ACIDS RESEARCH, 1991, 19 (22) :6191-6195

[10] MIGRATION PROPERTIES OF CIRCULAR DNAS USING ORTHOGONAL-FIELD-ALTERNATION GEL-ELECTROPHORESIS [J].

HIGHTOWER, RC ;

SANTI, DV .

ELECTROPHORESIS, 1989, 10 (5-6) :283-290

← 1 2 3 →