PERSISTENT EXPRESSION OF PROTOONCOGENE C-FOS STIMULATES OSTEOCLAST DIFFERENTIATION

被引：21

作者：

MIYAUCHI, A ^{[1
]}

KUROKI, Y ^{[1
]}

FUKASE, M ^{[1
]}

FUJITA, T ^{[1
]}

CHIHARA, K ^{[1
]}

SHIOZAWA, S ^{[1
]}

机构：

[1] KOBE UNIV, SCH MED, DEPT MED, DIV 3, KOBE 650, JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1994年 / 205卷 / 03期

关键词：

D O I：

10.1006/bbrc.1994.2843

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We analyzed c-fos mRNA expression by northern blotting analysis in chicken osteoclast precursors which spontaneously differentiate to multinucleated osteoclasts in 5-6 days. Osteoclast precursors as well as mature multinucleated osteoclasts showed constitutive expression of c-fos mRNA which is not found in osteoblasts. The c-fos expression was enhanced transiently by serum, dibutyryl cAMP (10(-4) M) and phorbol 12-myristate 13-acetate (TPA) (5 X 10(-7) M). To clarify the role of c-fos in osteoclast differentiation, c-fos DNA was transfected into osteoclast precursors. Greater than 2 fold increases in tartrate resistant acid phosphatase(TRAP) and bone resorptive activity were observed in the transfected cells compared to controls 3 days after transfection, suggesting that prolonged expression of c-fos caused enhanced osteoclast differentiation. (C) 1994 Academic Press, Inc.

引用

页码：1547 / 1555

页数：9

共 21 条

[1] GENERATION OF AVIAN CELLS RESEMBLING OSTEOCLASTS FROM MONONUCLEAR PHAGOCYTES [J].